IFN Regulatory Factors 4 and 8 Expression in the NOD Mouse
Figure 2
Age-related IRF4 and IRF8 expression levels in splenocytes of diabetes-prone NOD mice and diabetes-resistant BALB/c mice. Proteins were extracted from NOD or BALB/c mouse splenocytes and analyzed by Western blotting for IRF4 (a) or IRF8 (c) expression. The relative intensities of the bands were determined using the NIH Image software and normalized to reference actin bands to establish a ratio of (b) IRF4/actin and (d) IRF8/actin. The levels of expression in BALB/c mice were arbitrarily set at a unitary value. Data are representative of 3 independent experiments.
