Involvement of Indoleamine 2,3-Dioxygenase in Impairing Tumor-Infiltrating CD8+ T-Cell Functions in Esophageal Squamous Cell Carcinoma
Figure 4
Effect of IFNγ on IDO expression in the esophageal carcinoma cell lines. Esophageal carcinoma cell lines Kyse 140, TE-1, and Eca109 were treated with or without 100 U/mL IFNγ for 24 hr, and expression of the IDO protein was assessed by western blot (a). IFNγ induced IDO protein expression in Eca109 cells in a dose-dependent manner. Eca109 cells were cultured in the presence of indicated concentrations of IFNγ for 24 hr, IDO expression was analyzed by western blotting (b); the supernatants of Eca109 cells were analyzed for Kyn and Trp production using HPLC (c). Columns, mean of three independent experiments. Bars, SD.
