Anti-IL-6 Receptor Antibody Causes Less Promotion of Tuberculosis Infection than Anti-TNF-𝛼 Antibody in Mice

Figure 5

IFN-𝛾 production and proliferation of spleen cells obtained 4 weeks after TB infection from Ab-treated mice in response to TB antigens (PPD). (a) Spleen cells from each group (𝑛=5) were pooled and cultured with 20 𝜇g of PPD or TB antigens per mL for 48 h, and then IFN-𝛾 in the culture supernatant was measured. Results are the mean ± S.E.M of triplicate culture. *𝑃<.01 versus corresponding control by Student’s 𝑡-test. (b) Spleen cells were incubated in the presence of PPD, and BrdU incorporation in the nucleus was measured as O.D. ***(optical density at 340 nm) as an indicator of proliferation. Results are the mean ± S.E.M. of triplicate samples from 5 mice per group. *𝑃<.05 and **𝑃<.01 versus corresponding control by Student’s 𝑡-test.

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