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Journal of Immunology Research
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Journal of Immunology Research
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2011
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Article
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Tab 1
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Clinical Study
Upregulated Expression of Indoleamine 2, 3-Dioxygenase in Primary Breast Cancer Correlates with Increase of Infiltrated Regulatory T Cells
In Situ
and Lymph Node Metastasis
Table 1
Primers for real-time quantitative RT-PCR.
Gene name
Primer sequences
Product size
IDO
UP: 5′-CATCTGCAAATCGTGACTAAG-3′
188 bp
DP: 5′-CAGTCGACACATTAACCTTCCTTC-3′
Foxp3
UP: 5′-CCCACTTACAGGCACTCCTC-3′
486 bp
DP: 5′-CTTCTCCTTCTCCAGCACCA-3′
β
-actin
UP: 5′-TGGCACCCAGCACAATGAA-3′
186 bp
DP: 5′-CTAAGTCATAGTCCGC CTAGAAGCA-3′
Note: UP: upstream primer; DP: downstream primer.