Novel Prophylactic Vaccine Using a Prime-Boost Method and Hemagglutinating Virus of Japan-Envelope against Tuberculosis
Figure 3
(a) Mouse protection studies using the prime-boost method. Groups of mice vaccinated with HVJ-envelope (HVJ-E) DNA and/or BCG were challenged by intravenous injection with M. tuberculosis H37Rv. Five weeks after challenge, protection was measured by enumerating the bacterial loads (CFU) in the lungs. Results are expressed as the mean log10 ± S.D. of CFU. The statistical significance of differences between individual groups in the CFU number was determined by Dunnett test (); and ; the statistical significance of differences () of the G1 (naive) group compared to the G3 group (DNA/DNA/DNA), G4 group (BCG/DNA/DNA), or the G5 group (DNA/DNA/BCG). The statistical significance of differences () of the G2 group (BCG-alone group) compared to the G3 group (DNA/DNA/DNA), that of differences () of the G2 group compared to the G4 group (BCG/DNA/DNA), or the G5 group (DNA/DNA/BCG). (b) Mouse protection studies using the prime-boost method. Groups of mice vaccinated with HVJ-E DNA and/or BCG were challenged by intravenous injection with M. tuberculosis H37Rv. Five weeks after challenge, protection was measured by enumerating the bacterial loads (CFU) in the liver. Results are expressed as the mean log10 ± S.D. of CFU. The statistical significance of differences between individual groups in the CFU number was determined by Dunnett test (), ; the statistical significance of differences () of the G1 (naive) group compared to the G4 group (BCG/DNA/DNA), or the G5 group (DNA/DNA/BCG). The statistical significance of differences () of the G2 group (BCG-alone group) compared to the G4 group (BCG/DNA/DNA). The statistical significance of differences() of the G2 group compared to the G5 group (DNA/DNA/BCG). The statistical significance of differences () of the G3 group (DNA/DNA/DNA) compared to G4 (BCG/DNA/DNA). That of differences () of the G3 group compared to the G5 group. (c) Mouse protection studies using the prime-boost method. Groups of mice vaccinated with HVJ-E DNA and/or BCG were challenged by intravenous injection with M. tuberculosis H37Rv. Five weeks after challenge, protection was measured by enumerating the bacterial loads (CFU) in the spleen. Results are expressed as the mean log10 ± S.D. of CFU. The statistical significance of differences between individual groups in the number of CFU was determined by Dunnett test (); ; the statistical significance of differences () of the G1 (naive) group compared to the G4 group (BCG/DNA/DNA).
