549281.fig.008
Figure 8: ELISPOT assay for IFN-γ antigen-specific responses in the spleens of vaccinated mice following stimulation with rHSP65 protein. Spleen cell cultures were stimulated with rHSP65 protein for 20 h. The numbers of IFN-γ-secreting cells specific for rHSP65 protein per million cells were determined individually by ELISPOT assay. Results are expressed as the mean ± S.D. of 6 wells of 3 mice per group. The statistical significance of differences between individual groups in the number of IFN-γ-secreting cells was determined by Dunnett test. The statistical significance of differences ( ) of the G1 (naive) group compared to the G2 (BCG alone group), G3 (DNA/DNA/DNA), G4 (BCG/DNA/DNA), or G5 (DNA/DNA/BCG). The statistical significance of the G2 group difference ( ) compared to the G4 or the G5. The statistical significance of the G3 group differences ( ) compared to the G4. ; the G4 group compared to the G5. The statistical significance of the G3 group differences ( ) compared to the G5.