NOD Dendritic Cells Stimulated with Lactobacilli Preferentially Produce IL-10 versus IL-12 and Decrease Diabetes Incidence

Figure 3

Activation of NOD and B6 DCs with different strains of Lactobacilli in vitro. BM-DCs from NOD or control B6 mice were stimulated for 24 hrs with 10 × 10⁶ CFU/mL L. casei (LC), L. plantarum (LP), L. reuteri (LR), or medium alone (none). After 24 hrs, supernatants were collected and measured by ELISA for the presence of IL-10 or IL-12 (a and b), or cells were collected and labeled with anti-CD11c, anti-CD11b, and anti-B7-1 (c) or anti-B7-2 (d) antibodies, and analyzed by FACS after gating on CD11b⁺CD11c^int cells.

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