NOD Dendritic Cells Stimulated with Lactobacilli Preferentially Produce IL-10 versus IL-12 and Decrease Diabetes Incidence
Figure 4
Effect of different doses of L. casei on the profiles of IL-10 and IL-12 production by NOD DCs in vitro and disease incidence. BM-DCs from NOD mice were stimulated with either 1 × 106 CFU/mL (Low LC), 20 × 106 CFU/mL (High LC) of the L. casei or medium alone (none). After 24 hrs, supernatants were collected and measured by ELISA for the presence of IL-10 (white bar) or IL-12 (black bar) (a). Six-week-old NOD mice were left untreated or received a single i.v. injection of 1 × 106 DCs treated with either low (1 × 106 CFU/mL-low LC) or high (20 × 106 CFU/mL-high LC) dose of L. casei (LC) (–6) (b). Blood glucose was measured weekly and diabetes incidence determined. *indicates a significant difference at .
