NOD Dendritic Cells Stimulated with Lactobacilli Preferentially Produce IL-10 versus IL-12 and Decrease Diabetes Incidence

Figure 5

Injection of Lactobacilli-treated DCs into NOD mice confers some protection. Six-week-old NOD mice received two injections of 1 × 10⁶  DCs treated with high dose (20 × 10⁶ CFU/mL; NOD-high LC) of LC at 6 and 16 weeks of age (𝑛=4–6) and blood glucose was measured weekly and diabetes incidence determined (a). Sera from NOD mice uninjected or injected with DCs treated with high-dose LC (high LC) were collected at 28 hrs after injection, and tested for the presence of IL-10 by ELISA (b). Six-week-old NOD mice left uninjected or injected with 1 × 10⁶ untreated DCs (𝑛=4-5) or DCs treated with high dose of LC (𝑛=4) received anti-IL-10R or isotype control antibodies, and blood glucose was measured weekly and diabetes incidence determined (c). *indicates a significant difference at 𝑃<.03.

(a)

(b)

(c)