Review Article
Modulation of Cell Death by M. tuberculosis as a Strategy for Pathogen Survival
Table 1
Alteration in cell death by apoptosis or necrosis in THP-1 cells infected with BCG or M. tuberculosis Erdmann (MOI 50) assessed at 24 hours by the Cell Death Detection ELISAPLUS photometric enzyme immunoassay (Roche Diagnostics, Lewes, UK) which measures cell death by both apoptosis and necrosis on fractionated samples. Associated changes in mRNA for the major host genes involved in the activation of the extrinsic pathway of apoptosis were assessed by quantitative Real-Time PCR, using HuPO as a housekeeping gene for normalization. Results shown are relative to untreated cells, of the means of assays from a single experiment (representative of 4) performed in triplicate (ELISA) or quadruplicate (RT-PCR). Values marked in bold text represent a significant increase, those in italics a significant decrease. The ANOVA test (with Dunnett’s multiple comparison posttest for all groups against untreated controls) was used for analyses between groups. In all instances, a value was considered significant. A value of “<.1” indicates below the limit of detection, with “ND” indicates that the experiment was not done.
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