Research Article
The M. tuberculosis Phosphate-Binding Lipoproteins PstS1 and PstS3 Induce Th1 and Th17 Responses That Are Not Associated with Protection against M. tuberculosis Infection
Table 1
Effects of different immunizations with the mycobacterial antigen Ag85B on protection against Mycobacterium tuberculosis challenge and antigen-specific immunity in mice.
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amice (5 mice/group) were immunized with Ag85B according to Figure 1, and some mice challenged also with M. tuberculosis H37Rv. bsera of immunized mice recovered 4 weeks from the last immunizations were analyzed by ELISA for the presence of anti-Ag85B Ab using a conjugated secondary Abs specific total IgG. Data are plotted as geometric mean ELISA titer ± SEM of 3 independent experiments. cspleen cells of Ag85B-immunized mice were cultured at cells/well and re-stimulated ex vivo with Ag85B protein (5 μg/mL) for 5 days before measuring thymidine incorporation in proliferating cells. Cell proliferation data are represented as mean cpm (subtracted from the cpm of unstimulated spleen cells) ± SEM of 3 independent experiments. dspleen cells of Ag85B-immunized mice were cultured at cells/well and re-stimulated ex vivo with Ag85B protein (5 μg/mL) for 4 days before measuring in cell culture supernatants IFN-γ by a specific ELISA kit. IFN-γ data are represented as mean (pg/mL) ± SEM of 3 independent experiments. espleen cells of Ag85B-immunized mice were cultured at cells/well and re-stimulated ex vivo with Ag85B protein (5 μg/mL) for 4 days before measuring in cell culture supernatants IL-17 by a specific ELISA kit. IL-17 data are represented as mean (pg/mL) ± SEM of 3 independent experiments. fAfter 4 weeks from M. tuberculosis challenge, the bacteria in the lung of infected mice were enumerated as described in Materials and Methods. Data are expressed as the Δ in CFU/lung (log) between vaccinated and unvaccinated control mice and it is indicated whether or not the vaccination was protective against M. tuberculosis challenge. gReference reporting some of the data. |