Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector
Figure 1
The map of lentiviral vector pWPT-promotor-α/β chain and the schematic diagrams representing the structures of the lentiviral vectors. (a) The map of lentiviral vector pWPT-promotor-α/β chain. The promoter-α/β chain was amplified by PCR and inserted between Mlu I and Sal I sites. (b) The schematic diagrams representing the structures of the lentiviral vectors. All the α/β chains of the anti-LMP2 TCR PL5.05 were driven by individual T-cell-specific promoter except mock which contains only α or β chains without any promoter region. Promoter-α chains in diagram forms were the lentiviral vectors designed to express α chain driven by Vβ 5.1, Luria, Delta, and Vβ 6.7 promoters, respectively. Promoter-β chain used Vβ 5.1, Luria, Delta, and Vβ 6.7 promoters to produce the individual β chain.
