Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector
Figure 4
Transduced PBMCs specifically lyse HLA-A2-restricted LMP2-expressing target cells. (a) Lytic activity of CD8+ cells selected from PBMCs transduced with lentivirus containing LMP2-TCR at indicated effector-to-target cell ratios (E : T) was demonstrated in an LDH-release assay. Targets were HLA-A2-restricted T2 cells loaded with CLGG. Results are expressed as percent of the value measured in control cells incubated with the same volume of medium (mean ± SD of 3 replicates). Lytic activity of PBMCs (b) or CD8+ cells (d) selected from PBMCs transduced with lentivirus-containing LMP2-TCR driven by T-cell promoter Vβ 6.7 at indicated E : T was demonstrated in an LDH-release assay. Targets were HLA-A2-matched T2, T2-CLGG, LCL, T2-LLWT, and exceptional K562 cells. All figures are representative of 3 or more experiments using the same PBMC donor. (c) Levels of IFN-γ being released into the media from transduced PBMC effectors in the lysis assay above. PBMCs expressing LMP2-TCR were cocultured for 16 hr with the indicated target cells. The concentration of IFN-γ secreted into the medium was measured in an ELISA kit.
