Research Article

Recombinant HBHA Boosting Effect on BCG-Induced Immunity against Mycobacterium tuberculosis Infection

Figure 1

Spleen cell cytokine profile of BCG-vaccinated mice with or without rHBHA boost after M. tuberculosis infection. Eight weeks after i.n. (A) or aerosol (B) challenge spleen cells from control mice (white bars), BCG-vaccinated mice (black bars), or BCG-vaccinated/rHBHA-boosted mice (gray bars) were cultured in the presence of medium only, ConA (2.5 μg/mL), or rHBHA (5 μg/mL). Levels of cytokines were measured after 72 h culture in the supernatants by using the OptEIA kit (BD Biosciences). Values are expressed in pg/mL and represent media ± SEM of samples tested in duplicates from each group of mice. The data was considered statistically significant when (*), (**), and (***).
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