Research Article

Recombinant HBHA Boosting Effect on BCG-Induced Immunity against Mycobacterium tuberculosis Infection

Table 1

Differential recombinant HBHA boosting effect of BCG-induced protective immunity in BALB/c newborn mice against M. tuberculosis H37Rv infection as measured by reduced bacterial load in spleen and lungs.

Vaccine Lung CFU Lung protectionSpleen CFU Spleen protection
VersusVersus
ControlBCGControlBCG

Contro
BC 1.0* 0.89*
BCG-rHBH 2.26*1.26** 1.32*0.43**
Contro
BC 0.59* 0.63*
BCG-rHBH 0.59* 1.35*0.72**

aData are represented as mean ± standard deviations of the log10-transformed bacterial (CFUs) of M. tuberculosis per organ; bintranasal challenge; caerosol challenge. Protection is calculated with respect to control of vaccination (BCG) and with respect to PBS-immunized newborn mice (control). The data reported are ignificant at with respect to control mice and **significant at with respect to BCG control of vaccination with no boost. One representative of a total of two independent experiments is shown.