Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3
Figure 1
CD127 mRNA expression in CD4+CD25high Treg cells and integration of CD127 in the analysis of human Treg cells. (a) Expression of CD127 mRNA in CD4+CD25high Treg cells and conventional CD4+CD25− T cells in healthy donors (, control) and CLL patients (, CLL) as determined by qPCR (*, , Student’s t-test). (b) Gating strategies for analysis of expression of CD127 in CD4+CD25highFOXP3+ Treg cells or (c) CD25 expression in CD4+CD127lowFOXP3+ Treg cells.