Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3

Figure 4

Functional analysis of CD4⁺CD127^lowCD25⁺ T_reg cells in cancer patients. (a) Sorting strategy for isolating CD4⁺CD127^lowCD25⁺ T_reg cells (T_reg) as well as conventional CD4⁺CD127⁺CD25⁻ T cells (T_conv). (b) Expression of FOXP3 in the corresponding T-cell populations. (c) Percentage of proliferation of CD4⁺CD25⁻CD127⁺ T_conv cells (black bar) alone or cultivated with CD4⁺CD127^lowCD25⁺ T_reg cells derived from CRC patients (𝑛=4) at a 1 : 1 ratio (white bar) both in the presence of CD3/CD28 mAb coated beads. Error bars represent standard deviation (*, 𝑃<0.05, Student’s t test).

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