Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin

Figure 4

Analysis of RNA modulation by q-rt RT-PCR. Total RNA from DCs exposed for 3 h to MTB H37Rv, MTB CMT97, BCG Aventis and BCG Japan was assayed for gene expression by q-rt RT-PCR, 1-day culture after the infections. Gene expression was normalized to L34 and fold change was calculated with respect to uninfected DCs. Data are from 3 individual experiments and are expressed as mean ± SEM. The presence of significant differences in gene expression between DCs was calculated by a one-way ANOVA.