HLA Class II Defects in Burkitt Lymphoma: Bryostatin-1-Induced 17 kDa Protein Restores CD4+ T-Cell Recognition
Figure 4
BL and B-LCL bind synthetic peptide with similar affinity. Ag presentation depends on efficient binding of Ag to HLA class II proteins. BL cell lines Nalm-6.DR4 and Ramos.DR4, and the B-LCL lines 6.16.DR4.DM and Frev were fixed and incubated with biotin-labeled at pH 7.4. Cells were lysed and class II peptide complexes were detected in ELISA format with europium-labeled streptavidin with mean fluorescence used as a measure of peptide binding. Each cell line bound similar levels of peptide.
