Research Article

Different Pathological Roles of Toll-Like Receptor 9 on Mucosal B Cells and Dendritic Cells in Murine IgA Nephropathy

Figure 1

The disease course in each CpG-ODN group. (a) Urinary albumin in each group. Arrows indicate administration of each CpG-ODN agent. Urine samples were collected before and after administration. (b) Glomerular pathological changes at eight weeks. Glomerular damage in each CpG-ODN and vehicle control group were evaluated by PAS and Azan staining, and immunohistochemical analysis of α-SMA, type I collagen (Col. I), and type IV collagen (Col. IV). (c) Evaluation of cell proliferation and extracellular matrix (ECM) expansion in the glomeruli of each CpG-ODN group. Cell proliferation was evaluated by counting the average cell number in each glomerulus. ECM expansion was evaluated by the percentage of blue areas in each glomerulus stained by AZAN. In both experiments, more than 30 glomeruli were evaluated in each mouse. Magnification in (b): 400X.
819646.fig.001a
(a)
819646.fig.001b
(b)
819646.fig.001c
(c)