Research Article

Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein

Figure 8

Proliferation and migration abilities of splenocytes in treated NOD mice. Splenocytes (1 × 107 cells) isolated from CTB-INS-, CTB-Ins-GFP-, CTB-GFP-, Saline-fed and normal mice at 10 weeks of age were cultured in 24-well plates with 10 μM BrdU for 24 h. Cells were immunohistochemically stained and examined for stained cell proportions (representative of proliferative ability) according to the protocol. Or splenocytes were cultured in the upper chamber of Transwell plates with 0.6 mL of normal culture medium (IL2 150 IU/mL) placed in the bottom chamber at 37°C with 5% CO2 for 14 h. The migrated cells were fixed, stained, and numbered under the microscope. A. Splenocyte BrdU assay. Magnification is 400x. (b) Splenocyte Transwell assay. The magnification is 400x. (c) Statistical analysis of proliferative ability. (d) Statistical analysis of migratory ability. All results are presented as the mean titer values ± SD. Five mice per group were tested in two separate experiments.
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