Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein
Figure 9
Splenocytes from insulin-fed NOD mice prevented diabetes after adoptive transfer. Splenocytes (1 × 107 cells) isolated from CTB-INS- (group 1, black), CTB-Ins-GFP- (group 2, red), and CTB-GFP- (group 3, green) fed mice at 10 weeks of age were mixed with diabetogenic splenocytes (1 × 107 cells) from diabetic NOD mice, and then cotransferred to 8-week-old NOD/SCID mice ( for all four groups) with a diabetogenic splenocyte-only injection (group 4, blue) as a control. The development of diabetes in the recipients was monitored for 15 weeks. The ANOVA test revealed a significant reduction in T1D onset in groups 1 and 2 compared with groups 3 and 4 (). No significant difference was found between groups 3 and 4 ().
