Research Article

Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein

Table 2

Treg cell proportion in the peripheral lymph tissues of NOD mice.

Type of miceMaterial oral administratedCD4+CD25+Foxp3+ T cell's proportion (%)
NondiabetesDiabetes
SpleenLymph nodeBloodSpleenLymph nodeBlood

NOD miceSaline1.45 ± 0.381.82 ± 0.362.22 ± 0.145.42 ± 0.838.11 ± 1.620.63 ± 5.79
CTB-GFP1.54 ± 0.421.93 ± 0.292.19 ± 0.2
CTB-Ins-GFP2.75 ± 0.34*2.9 ± 0.42*3.61 ± 0.70*
CTB-INS2.87 ± 0.35****3.16 ± 0.53*3.71 ± 0.78*

Normal mice0.89 ± 0.11 ± 0.281.5 ± 0.36

Splenocyte (1 × 105 cells) isolated from CTB-INS, CTB-Ins-GFP, CTB-GFP, Saline-fed NOD mice at 10 weeks of age were treated with the mouse regulatory T cell staining kit by the protocol. Splenocytes of the normal mice and the diabetic NOD mice (from the diabetes onset experiment) was used as contrasts. These treated splenocytes were examined by FACScan flow cytometer for CD4+CD25+Foxp3+ Treg cell proportions. Results are presented as the mean titer values ± S.D. Four mice per group were tested in two separate experiments.
*Values is for statistical differences among mice groups treated with CTB-INS, CTB-Ins-GFP, CTB-GFP, and Saline.