Tumor and Microenvironment Modification during Progression of Murine Orthotopic Bladder Cancer

Table 1

Primer sequences, annealing temperature, and fragment length of PCR products.

Gene	Primer sequence (5′–3′)	Temp. (°C)	Size (bp)
ACTB F	ACATGGAGAAGATCTGGCAC	58	660
ACTB R	CAGACAGCACTGTGTTGGCA
CCL2 F	GCATCCACGTGTTGGCTCAG	60	383
CCL2 R	CACACTGGTCACTCCTACAG
CCL22 F	CGTCCTTCTTGCTGTGGCAA	60	233
CCL22 R	CTTCTTCACCCAGACCTGCC
CCL3 F	GCAACCAAGTCTTCTCAGCG	58	194
CCL3 R	CTTGGACCCAGGTCTCTTTG
CCL5 F	GGTACCATGAAGATCTCTGC	51.5	286
CCL5 R	CTATCCTAGCTCATCTCC
CCR2 F	GAGCCTGATCCTGCCTCTAC	58	371
CCR2 R	GGCACTGTTTGAAGAGACGT
CD80 F	GCAGGATACACCACT	55	480
CD80 R	GGAAGCAAAGCAGG
CSF2 F	TGGCCTGGGCTTCCTCAT	60	311
CSF2 R	GGATGACATGCCTGTCAC
CXCL10 F	CGTGGTCACATCAGCTGCTA	58	244
CXCL10 R	TAGAACTGACGAGCCTGAGC
CXCL11 F	AGGTCACAGCCATAGCCCTG	62	251
CXCL11 R	CCTGCATTATGAGGCGAGCTTGC
FOXP3 F	TCGTAGCCACCAGTACTCAG	57	386
FOXP3 R	ATCTACGGTCCACACTGCTC
GAPDH F	CACCCTGTTGCTGTAG	60	900
GAPDH R	GTCGGTGTGAACGGAT
GATA3 F	GATAGCATGAAGCTGGAGACG	60	500
GATA3 R	AAGCTTGTAGTACAGCCCACA
IFNg F	ACTGCCACGGCACAGTC	60	389
IFNg R	CCGCTTCCTGAGGCTG
IL12a F	CCATCGATGAGCTGATGCAG	58	340
IL12a R	ATGCTGAGGTAGCTGTGCCA
IL13 F	TGTCTCTCCCTCTGACCC	60	201
IL13 R	TACAGAGGCCATGCAATATCC
IL15 F	ATGAACTGCTTTCTCCTGGAA	60	205
IL15 R	TGGACAATGCGTATAAAGCTTTGC
IL17 F	CCAGGGAGAGCTTCATCTGT	58	431
IL17 R	AAGATGCTGGTGGGTGTGGG
IL1b F	GGCAACTGTTCCTGAACTCAACTG	62	739
IL1b R	GCTTGTCTGCTGCTTGTGAGGTGC
IL2 F	TGATGGACCTACAGGAGCTCCTGAG	60	191
IL2 R	GAGTCAAATCCAGAACATGCCGCAG
IL6 F	GATGCAACCAAACTGGATATAATC	60	225
IL6 R	GAGCATTGGAAGTTGGGGTA
NOS2 F	AAAGCCACGAGGCTCTGACA	58	259
NOS2 R	ACCATCACGCTCGAGGTTGA
PTGS2 F	ATGCTCTTCCGAGCTGTGCT	55	239
PTGS2 R	GTGGGTCAGGATGTAGTGCA
TBET F	CTAAAGCTCACCAACAACAAG	55	840
TBET R	GTTGGGAAAATAATTATAAAA
TGFb1 F	CTGCAAGACCATCGACAT	55	580
TGFb1 R	ACAAGAGCAGTGAGCGCT
TNF F	TGCACCACCATCAAGGACTC	58	360
TNF R	CAGCTCAGCTCCGTTTTCAC