Tumor and Microenvironment Modification during Progression of Murine Orthotopic Bladder Cancer
Table 2
Relative expression of genes in C57BL/6 mice bearing bladder tumors.
Day 7
Day 28
Gene
Control (7)
Tumor (12)
Control (11)
Cured (4)
Tumor (15)
Realtime†
AGTR2
0.871 ± 0.123
0.231 ± 0.250*
1.381 ± 0.623
1.072 ± 0.251
0.171 ± 0.205*
CSF2
0.944 ± 0.218
26.07 ± 28.00*
2.621 ± 5.554
1.637 ± 0.443
38.10 ± 37.28*
CTLA4
1.088 ± 0.567
38.27 ± 36.06*
2.421 ± 6.216
3.026 ± 0.834
47.19 ± 30.92*
GZMB
0.927 ± 0.312
27.07 ± 25.39*
0.966 ± 0.291
2.495 ± 0.456
7.349 ± 7.158*
IFNg
1.178 ± 0.455
127.5 ± 127.9*
0.726 ± 0.542
2.005 ± 0.742
8.875 ± 10.89*
IL10
5.022 ± 8.895
17.94 ± 36.01
1.377 ± 0.981
2.526 ± 0.975
3.749 ± 2.838*
PTGS2
1.126 ± 0.781
2.424 ± 0.789*
1.245 ± 0.844
3.818 ± 3.132
3.040 ± 6.290
TGFb1
0.934 ± 0.302
2.450 ± 2.500
1.251 ± 0.576
3.885 ± 3.301
2.110 ± 1.439
TNF
0.956 ± 0.155
11.36 ± 12.66*
2.658 ± 5.417
3.333 ± 2.303
8.008 ± 14.67
PSA
2.157 ± 1.898
126.7 ± 163.7*
0.491 ± 0.532
0.095 ± 0.099
243.4 ± 258.1*
RT-PCR
CCL2
1.140 ± 0.510
2.200 ± 0.840
0.799 ± 0.398
1.350 ± 0.616
1.996 ± 0.630*
CCL22
0.063 ± 0.026
0.212 ± 0.062*
0.095 ± 0.019
0.139 ± 0.050
0.108 ± 0.070
CCL3
0.193 ± 0.340
0.073 ± 0.044
0.928 ± 0.397
1.699 ± 2.210
2.366 ± 1.533
CCL5
0.308 ± 0.206
0.613 ± 0.284
0.360 ± 0.050
0.670 ± 0.170
0.680 ± 0.090*
CD80
0.094 ± 0.055
0.367 ± 0.094*
0.016 ± 0.008
0.134 ± 0.268
0.631 ± 0.825
CXCL10
2.383 ± 1.339
591.9 ± 257.8*
1.854 ± 1.257
4.831 ± 2.943
17.04 ± 30.28
CXCL11
0.523 ± 0.351
1.445 ± 0.374
0.297 ± 0.390
0.854 ± 0.656
2.240 ± 2.576*
FOXP3
0.016 ± 0.007
0.217 ± 0.203
0.042 ± 0.018
0.068 ± 0.058
0.155 ± 0.141
GATA3
0.901 ± 0.327
0.118 ± 0.039*
0.858 ± 0.173
0.935 ± 0.203
0.584 ± 0.244
IL12a
0.014 ± 0.008
0.064 ± 0.031*
—
—
—
IL13
0.020 ± 0.010
0.600 ± 0.220*
0.118 ± 0.277
0.060 ± 0.063
0.033 ± 0.013
IL15
1.295 ± 0.685
0.773 ± 0.381
0.298 ± 0.149
0.218 ± 0.255
0.225 ± 0.189
IL1b
0.125 ± 0.230
1.174 ± 0.578*
0.006 ± 0.003
0.011 ± 0.005
0.211 ± 0.177
IL2
0.681 ± 0.714
0.994 ± 0.609
2.098 ± 1.635
1.764 ± 0.804
1.582 ± 1.329
IL6
1.550 ± 0.670
3.190 ± 0.710*
0.829 ± 0.099
1.482 ± 1.026
4.957 ± 2.054*
NOS2
0.580 ± 0.100
0.950 ± 0.230*
—
—
—
TBET
0.057 ± 0.009
0.051 ± 0.013
0.083 ± 0.068
0.097 ± 0.068
0.075 ± 0.035
Note. The sample size for each group is indicated in brackets and “—” indicates not done. †Data is presented as the mean RQ ± SD. *indicates with respect to control.
