Antitumor Immunity Produced by the Liver Kupffer Cells, NK Cells, NKT Cells, and CD8⁺ CD122⁺ T Cells

Table 1

NKT cells are IL-12-induced antimetastatic effectors.

Mouse strain/treatment	Tumor	Site of metastasis tested	Number of tumor metastases
			Control	IL-12 treated	% inhibition
BALB/c + IL-12	RL♂1	liver	216 ± 24	28 ± 2	87%*
	Colon 26	lung	125 ± 25	16 ± 10	87%*
DBA/2 + lL-12	P815	liver	173 ± 12	10 ± 1	94%*
C57BL/6 + IL-12	B16	lung	61 ± 16	5 ± 1	91%*
	EL4	liver	106 ± 22	17 ± 7	84%*
C57BL/6 bg/bg + IL-12	EL4	liver	107 ± 17	16 ± 6	85%*
C57BL/6 + IL-12	EL4	liver	96 ± 18	15 ± 4	84%*
	3LL	lung	122 ± 26	25 ± 5	80%*
C57BL/6 + 𝛼AGM1 Ab + IL-12	EL4	liver	102 ± 24	22 ± 5	78%*
	3LL	lung	128 ± 32	33 ± 8	74%*
C57BL/6 + 𝛼NK1.1 Ab + IL-12	EL4	liver	152 ± 26	130 ± 20	14%
	3LL	lung	204 ± 36	156 ± 28	24%

The mice were inoculated i.v. with syngeneic tumors. Data of tumor metastasis and % of inhibition are shown as mean ± SD from six to ten mice in each group. ^∗𝑃<.01. αAGM1 Ab: antiasialo GM1 antibody; αNK1.1 Ab: anti-NK1.1 antibody.