Immunological and Nonimmunological Effects of Indoleamine 2,3-Dioxygenase on Breast Tumor Growth and Spontaneous Metastasis Formation

Figure 3

Flow cytometry analysis of apoptosis and cell cycle in Ido1+ and Ido1− cells. 4T1 and 4T1/Ido1− cells were cultured in normal medium (10% FBS), or under starvation condition (0.3% FBS) for 3 days followed by a 24 hour recovery in normal medium. Flow cytometry analyses of cells stained with FITC-conjugated antibromodeoxyuridine (BrdU) and propidium iodide (PI) were performed. The data are presented as a percentage of cells in apoptosis (A), G0, S, and M phases.