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Figure 4: Contribution of the C5aR-RP S19 oligomer system to the haemin-induced terminal differentiation of K562 cells under monocell culture conditions. K562 cells expressing EGFP-ANXA3/Mock, EGFP-ANXA3/Wild-type RP S19, EGFP-ANXA3/Q137N mutant RP S19, and EGFP-ANXA3/RGS3 were harvested at several time points after the haemin induction and analysed under CLSM ( ). The mock (black), Wild-type RP S19 (red), Q137N mutant RP S19 (green), and RGS3 (yellow) K562 cells were harvested at several time points after the haemin induction and stained with FITC-conjugated anti-CD71 and APC-conjugated anti-CD235a mouse IgGs for FACS analyses ( ). The averages of the percentage and number of CD235a+/CD71 erythrocyte-like cells were calculated on day 20 of the monocell culturing. values of less than 0.05 were considered statistically significant (* or ** ).