Dual Functions of the C5a Receptor as a Connector for the K562 Erythroblast-Like Cell-THP-1 Macrophage-Like Cell Island and as a Sensor for the Differentiation of the K562 Erythroblast-Like Cell during Haemin-Induced Erythropoiesis
Figure 7
Detection of the inhibition of the K562-THP-1 cell interactions by the inhibitors of the C5aR-RP S19 oligomer system using time-lapse microscopy. ((a)–(e)) EGFP-ANXA3-overexpressing K562 cells were cultured with the THP-1 macrophage-like cells in the presence of control rabbit IgG, anti-C5a rabbit IgG, PMX-53, W-54011, or SB 290157 for 1–3 days and were recorded using the LCV110 microscope (). The maximal number of cells containing haemoglobin-like molecules at 72 hr of coculturing was counted automatically from 4 random fields, and the averages were calculated. values of less than 0.05 were considered statistically significant (* or **).
