Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2

Table 1

Predicted antigenicity (antigen value) of the 20 epitopes and the EIA detection results.

Number	Predicted epitope	Surface probability	Flexibility	β-turn	Coil	Glycosylation site Asn-X-Thr/Ser	Predicted antigen value	EIA A450 value
(1)	gB258–75	5.219	44–82	69, 71	69, 70		1.588	0.361±0.011
(2)	gB2466–473	5.685	465–485	473	470		2.31	𝟎.𝟗𝟖𝟕±𝟎.𝟎𝟑𝟏
(3)	gB2468–475	4.185	465–475	473	470	+	1.593	0.276±0.011
(4)	gB2300–306	1.984	300–306	301	301–305		2.057	0.118±0.024
(5)	gC2326–335	1.320	328–335	330–332	333–335	+	2.044	0.278±0.011
(6)	gC295–105	1.315	95–100	93, 98	100–104		2.01	0.687±0.022
(7)	gC2161–171	1.948	161–170	167	164–170	+	1.736	0.689±0.021
(8)	gC2216–223	2.242	216–223	217, 222	219–223		2.235	𝟎.𝟖𝟗𝟖±𝟎.𝟎𝟑𝟎
(9)	gE2514–522	3.446	514–522	519	515		2.322	0.655±0.030
(10)	gE2471–479	2.02	471–479	478	471–47		2.287	0.877±0.031
(11)	gE2483–491	2.972	484–495	488, 492	485, 493		2.367	𝟏.𝟏𝟎𝟕±𝟎.𝟎𝟒𝟐
(12)	gE2385–398	2.701	385–398	396	385–394		2.49	0.564±0.021
(13)	gG2526–539	2.613	526–537	535, 539	530, 534		1.866	0.456±0.019
(14)	gG2286–295	1.215	289–293	290	288		1.56	0.821±0.025
(15)	gG2572–579	2.767	569–577	569, 574	570		2.807	𝟎.𝟖𝟏𝟏±𝟎.𝟎𝟑𝟏
(16)	gG2350–364	1.844	350–364	351	353–358		2.124	0.992±0.033
(17)	gI2202–226	2.453	202–226	203, 210, 217	205	+	1.859	0.413±0.011
(18)	gI2236–253	2.143	236–253	242, 253	241, 244–9, 251		2.081	0.856±0.026
(19)	gI2286–295	4.729	287–295	287, 293			2.764	𝟏.𝟎𝟑𝟒±𝟎.𝟎𝟑𝟗
(20)	gI2319–337	3.427	319–337	323, 326, 333	321, 325, 335		2.042	0.569±0.021
C								0.121±0.011