Research Article

Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2

Table 3

Antigenicity and specificity detection of the five peptides using Western Blot analysis.

NumberAntigen (peptides-BSA)Antibody to the proteins (titer)Western blottingAntigen (proteins)Antibody to peptides-BSA (titer)Western blotting

(1)gB2466–473/BSAgB2
(1 : 10 000)
++*gB2gB2466–473/BSA (1 : 5000)++
(2)gC2216–223/BSAgC2
(1 : 10 000)
++gC2gC2216–223/BSA (1 : 5000)++
(3)gE2483–491/BSAgE2
(1 : 10 000)
+++gE2gE2483–491/BSA (1 : 5000)+++
(4)gG2572–579/BSAgG2
(1 : 10 000)
++gG2gG2572–579/BSA (1 : 5000)++
(5)gI2286–295/BSAgI2
(1 : 10 000)
++gI2gI2286–295/BSA (1 : 5000)++
(6)BSAAll the proteins# (1 : 10 000)All the proteinsBSA**
(1 : 5000)

*: +++, strong reaction; ++, good reaction; +, moderate reaction; —, no reaction.
#: BSA did not react with all the antisera to the five proteins.
**: The five proteins did not react with the antisera to BSA.