Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2
Table 3
Antigenicity and specificity detection of the five peptides using Western Blot analysis.
Number
Antigen (peptides-BSA)
Antibody to the proteins (titer)
Western blotting
Antigen (proteins)
Antibody to peptides-BSA (titer)
Western blotting
(1)
gB2466–473/BSA
gB2 (1 : 10 000)
++*
gB2
gB2466–473/BSA (1 : 5000)
++
(2)
gC2216–223/BSA
gC2 (1 : 10 000)
++
gC2
gC2216–223/BSA (1 : 5000)
++
(3)
gE2483–491/BSA
gE2 (1 : 10 000)
+++
gE2
gE2483–491/BSA (1 : 5000)
+++
(4)
gG2572–579/BSA
gG2 (1 : 10 000)
++
gG2
gG2572–579/BSA (1 : 5000)
++
(5)
gI2286–295/BSA
gI2 (1 : 10 000)
++
gI2
gI2286–295/BSA (1 : 5000)
++
(6)
BSA
All the proteins# (1 : 10 000)
—
All the proteins
BSA** (1 : 5000)
—
*: +++, strong reaction; ++, good reaction; +, moderate reaction; —, no reaction. #: BSA did not react with all the antisera to the five proteins.
**: The five proteins did not react with the antisera to BSA.