Research Article

Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance

Figure 3

Effects of the treatments with OVA on the frequency of CD103+ cells in the small intestine. Freshly isolated IELs from BALB/c (a) and DO11.10 (b) mice were gated for CD3+/CD4+, CD3+/CD8α +, and CD3+/CD8β + cells and analyzed for expression of CD103 by flow cytometry. Blank histograms indicate isotype control staining. CD3+/CD8β + subset illustrated at the right column is part of the CD3+/CD8α + population. (c) Data represent mean ± SEM ( ) in three independent experiments. Frequency of CD4+ cells were significantly more elevated in IELs isolated from mice BALB/c and DO11.10 treated with OVA by oral + ip routes. The expression of CD103 was markedly augmented in all subpopulations of IELs of OVA-treated BALB/c mice and was reduced in DO11.10 immunized by ip route.
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