Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance

Figure 5

Effects of the treatments with OVA on the frequency of Foxp3⁺ cells in the small intestine. IELs were isolated from BALB/c (a) and DO11.10 (b) mice, and the frequency of Foxp3⁺ cells were analyzed on for CD3⁺/CD4⁺, CD3⁺/CD8α ⁺, and CD3⁺/CD8β ⁺ cells. (c) Data represent mean ± SEM (𝑁=5), in three independent experiments. Treatments of BALB/c mice with OVA resulted in increase of the frequency of Foxp3⁺ cells in all IEL populations. The oral + ip treatment of DO11.10 mice has increased the frequency of Foxp3⁺cells in IELs CD25⁺.

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