Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells

Figure 1

CIK cells are equally activated upon binding to CEA⁺ and CEA⁻ tumour cells. CIK cells (4×10⁴ cells) generated from blood lymphocytes of healthy donors were coincubated with CEA⁺ Colo205 or CEA⁻ Colo201 tumour cells (10³ tumour cells) for 48 hrs. IFN-γ production was monitored by ELIspot analysis. Data represent the mean ± standard error of the mean; a representative experiment out of three is shown. *𝑃<0.05.