Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells
Figure 4
Activation of CIK cells from cancer patients against autologous colon carcinoma cells. Primary colon carcinoma cells were isolated from a colon carcinoma biopsy as described in Section 2 and cultured in vitro for short term. CIK cells from the same patient were generated in vitro and engineered with the ζ and the CD28-ζ CAR. CIK cells were coincubated for 48 hrs with the autologous CEA+ colon carcinoma cells ( CIK cells; 103 tumour cells). Secreted IFN-γ in the culture supernatant was monitored by ELISA. Data represent the means of triplicates ± standard error of the mean. One representative experiment out of three is shown. *.
