Reintroduction of Gluten Following Flour Transamidation in Adult
Celiac Patients: A Randomized, Controlled Clinical Study
Figure 4
Assessment of intestinal mucosa following a 90-day (K-CH3)-transamidated gluten challenge. (a) Morphometric and immunohistochemical analyses of biopsy specimens from consenting patients () who completed the 90-day study without developing symptoms or altered permeability; the density of CD3+ cells in the intraepithelial compartment was determined by counting the number of stained cells as a percentage of 100 enterocytes; bars indicate medians, and the statistical evaluation of data was performed using the Wilcoxon signed-rank test. (b) IFN- mRNA levels in intestinal biopsies from patients in the experimental group () and from untreated CD patients () were evaluated by real-time PCR; the cytokine values were normalized to L-32 mRNA and are presented as fold change in gene expression (AU); bars indicate medians, and the Kruskal-Wallis statistic and Dunn’s Multiple Comparison test were used to compare differences among groups (1) *: ; **: .
