Combination Immunotherapy with 4-1BBL and CTLA-4 Blockade for the Treatment of Prostate Cancer
Figure 3
Cytotoxicity assay. Spleens were harvested from mice rejecting RM-1 cells and restimulated in vitro with MMC-treated RM-1 cells. 7 d later, cells were collected and purified by Ficoll-Histopaque gradient centrifugation as effector cells for detecting specific cytotoxicity against target cells. The data are expressed as means ± SD of three replicates ().
