Adaptive Immune Response to Model Antigens Is Impaired in Murine Leukocyte-Adhesion Deficiency-1 Revealing Elevated Activation Thresholds In Vivo
Figure 2
Functional humoral immune response upon NP-CG in CD18−/− mice. Eight- to twelve-week-old CD18−/− and WT mice were immunized intraperitoneally with 100 μg of alum-precipitated NP-CG. Animals were reinjected with 100 μg of soluble NP-CG at day 34. Sera were collected at days 0, 7, and 14 during primary immune response, and at days 34, 42, 49, and 56 during secondary immune response. (a) Serum levels of anti-NP-specific IgG1 were subsequently detected by ELISA on NP4-coated ELISA plates and calculated by comparison to an IgG1 standard. (b) Anti-NP-specific Ig carrying λ or κ L chains were differentially detected on high-density (NP14-BSA) and low-density (NP4-BSA) hapten-coated ELISA plates in sera obtained at days 14, 34, and 56. Affinity maturation of NP-specific antibodies was estimated as ratio of NP4- to NP14-binding antibodies for each of the three time-points. Bars represent the median of each group. **.
