About this Journal Submit a Manuscript Table of Contents
Clinical and Developmental Immunology
Volume 2012 (2012), Article ID 494356, 6 pages
http://dx.doi.org/10.1155/2012/494356
Review Article

The Clinical Significance of the Dense Fine Speckled Immunofluorescence Pattern on HEp-2 Cells for the Diagnosis of Systemic Autoimmune Diseases

1INOVA Diagnostics, Inc., 9900 Old Grove Road, San Diego, CA 32131-1638, USA
2Department of Medicine, Faculty of Medicine, University of Calgary, Calgary, AB, Canada T3H 1H7

Received 12 October 2012; Accepted 1 November 2012

Academic Editor: Dimitrios P. Bogdanos

Copyright © 2012 Michael Mahler and Marvin J. Fritzler. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Mahler and M. J. Fritzler, “Epitope specificity and significance in systemic autoimmune diseases,” Annals of the New York Academy of Sciences, vol. 1183, pp. 267–287, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. G. J. Friou, S. C. Finch, and K. D. Detre, “Interaction of nuclei and globulin from lupus erythematosis serum demonstrated with fluorescent antibody,” The Journal of Immunology, vol. 80, no. 4, pp. 324–329, 1958. View at Scopus
  3. A. E. Moore, L. Sabachewsky, and H. W. Toolan, “Culture characteristics of four permanent lines of human cancer cells,” Cancer Research, vol. 15, no. 9, pp. 598–602, 1955.
  4. P. L. Meroni and P. H. Schur, “ANA screening: an old test with new recommendations,” Annals of the Rheumatic Diseases, vol. 69, no. 8, pp. 1420–1422, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. M. J. Fritzler and M. L. Fritzler, “The emergence of multiplexed technologies as diagnostic platforms in systemic autoimmune diseases,” Current Medicinal Chemistry, vol. 13, no. 21, pp. 2503–2512, 2006. View at Publisher · View at Google Scholar · View at Scopus
  6. S. Kivity, B. Gilburd, N. Agmon-Levin, et al., “A novel automated indirect immunofluorescence autoantibody evaluation,” Clinical Rheumatology, vol. 31, no. 3, pp. 503–509, 2012. View at Publisher · View at Google Scholar
  7. K. Egerer, D. Roggenbuck, R. Hiemann et al., “Automated evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell-based immunofluorescence tests,” Arthritis Research & Therapy, vol. 12, no. 2, article R40, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Mahler, J. T. Ngo, J. Schulte-Pelkum, T. Luettich, and M. J. Fritzler, “Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies,” Arthritis Research & Therapy, vol. 10, no. 6, article R131, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. M. J. Fritzler, “The antinuclear antibody test: last or lasting gasp?” Arthritis & Rheumatism, vol. 63, no. 1, pp. 19–22, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. S. S. Copple, A. D. Sawitzke, A. M. Wilson, A. E. Tebo, and H. R. Hill, “Enzyme-linked immunosorbent assay screening then indirect immunofluorescence confirmation of antinuclear antibodies,” American Journal of Clinical Pathology, vol. 135, no. 5, pp. 678–684, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. K. Op De Beeck, P. Vermeersch, P. Verschueren et al., “Detection of antinuclear antibodies by indirect immunofluorescence and by solid phase assay,” Autoimmunity Reviews, vol. 10, no. 12, pp. 801–808, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. H. A. Mariz, E. I. Sato, S. H. Barbosa, S. H. Rodrigues, A. Dellavance, and L. E. C. Andrade, “Pattern on the antinuclear antibody-HEp-2 test is a critical parameter for discriminating antinuclear antibody-positive healthy individuals and patients with autoimmune rheumatic diseases,” Arthritis & Rheumatism, vol. 63, no. 1, pp. 191–200, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Watanabe, M. Kodera, K. Sugiura et al., “Anti-DFS70 antibodies in 597 healthy hospital workers,” Arthritis & Rheumatism, vol. 50, no. 3, pp. 892–900, 2004. View at Publisher · View at Google Scholar · View at Scopus
  14. R. L. Ochs, Y. Muro, Y. Si, H. Ge, E. K. L. Chan, and E. M. Tan, “Autoantibodies to DFS 70 kd/transcription coactivator p75 in atopic dermatitis and other conditions,” The Journal of Allergy and Clinical Immunology, vol. 105, no. 6, pp. 1211–1220, 2000. View at Publisher · View at Google Scholar · View at Scopus
  15. V. Ganapathy and C. A. Casiano, “Autoimmunity to the nuclear autoantigen DFS70 (LEDGF): what exactly are the autoantibodies trying to tell us?” Arthritis & Rheumatism, vol. 50, no. 3, pp. 684–688, 2004. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Daniels, J. Zhang, I. Gutierrez et al., “Antinuclear autoantibodies in prostate cancer: immunity to LEDGF/p75, a survival protein highly expressed in prostate tumors and cleaved during apoptosis,” Prostate, vol. 62, no. 1, pp. 14–26, 2005. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Mahler, T. Parker, C. L. Peebles, et al., “Anti-DFS70/LEDGF antibodies are more prevalent in healthy individuals compared to patients with systemic autoimmune rheumatic diseases,” The Journal of Rheumatology, vol. 39, no. 11, pp. 2104–2110, 2012. View at Publisher · View at Google Scholar
  18. A. Dellavance, V. S. T. Viana, E. P. Leon, E. S. D. O. Bonfa, L. E. C. Andrade, and P. G. Leser, “The clinical spectrum of antinuclear antibodies associated with the nuclear dense fine speckled immunofluorescence pattern,” The Journal of Rheumatology, vol. 32, no. 11, pp. 2144–2149, 2005. View at Scopus
  19. Y. Muro, K. Sugiura, Y. Morita, and Y. Tomita, “High concomitance of disease marker autoantibodies in anti-DFS70/LEDGF autoantibody-positive patients with autoimmune rheumatic disease,” Lupus, vol. 17, no. 3, pp. 171–176, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. Y. Muro, Y. Ogawa, K. Sugiura, and Y. Tomita, “HLA-associated production of anti-DFS70/LEDGF autoantibodies and systemic autoimmune disease,” Journal of Autoimmunity, vol. 26, no. 4, pp. 252–257, 2006. View at Publisher · View at Google Scholar · View at Scopus
  21. R. L. Ochs, T. W. Stein Jr., C. L. Peebles, R. F. Gittes, and E. M. Tan, “Autoantibodies in interstitial cystitis,” Journal of Urology, vol. 151, no. 3, pp. 587–592, 1994. View at Scopus
  22. N. Bizzaro, E. Tonutti, and D. Villalta , “Recognizing the dense fine speckled/lens epithelium-derived growth factor/p75 pattern on HEp-2 cells: not an easy task! Comment on the article by Mariz et al,” Arthritis & Rheumatism, vol. 63, no. 12, pp. 4036–4037.
  23. T. Shinohara, D. P. Singh, and L. T. Chylack Jr., “Review: age-related cataract: immunity and lens epithelium-derived growth factor (LEDGF),” Journal of Ocular Pharmacology and Therapeutics, vol. 16, no. 2, pp. 181–191, 2000. View at Scopus
  24. G. Maertens, P. Cherepanov, W. Pluymers et al., “LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells,” The Journal of Biological Chemistry, vol. 278, no. 35, pp. 33528–33539, 2003. View at Publisher · View at Google Scholar · View at Scopus
  25. M. Mahler, J. G. Hanly, and M. J. Fritzler, “Importance of the dense fine speckled pattern on HEp-2 cells and anti-DFS70 antibodies for the diagnosis of systemic autoimmune diseases,” Autoimmunity Reviews, vol. 11, no. 9, pp. 642–645, 2012. View at Publisher · View at Google Scholar
  26. N. Bizzaro, E. Tonutti, D. Visentini et al., “Antibodies to the lens and cornea in anti-DFS70-positive subjects,” Annals of the New York Academy of Sciences, vol. 1107, pp. 174–183, 2007. View at Publisher · View at Google Scholar · View at Scopus
  27. E. M. Tan, A. S. Cohen, and J. F. Fries, “The 1982 revised criteria for the classification of systemic lupus erythrematosus,” Arthritis & Rheumatism, vol. 25, no. 11, pp. 1271–1277, 1982. View at Scopus
  28. S. Narain, H. B. Richards, M. Satoh et al., “Diagnostic accuracy for lupus and other systemic autoimmune diseases in the community setting,” Archives of Internal Medicine, vol. 164, no. 22, pp. 2435–2441, 2004. View at Publisher · View at Google Scholar · View at Scopus
  29. M. R. Arbuckle, M. T. McClain, M. V. Rubertone et al., “Development of autoantibodies before the clinical onset of systemic lupus erythematosus,” The New England Journal of Medicine, vol. 349, no. 16, pp. 1526–1533, 2003. View at Publisher · View at Google Scholar · View at Scopus