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Figure 2: Dose-dependent effect of fresh collected maggot ES on activation of the classical pathway (white bars), the alternative pathway (grey bars), and the lectin pathway (black bars) in normal human sera. The complement activation in four different sera was determined with the enzyme immunoassays from Wieslab (EuroDiagnostica BV, Arnhem, The Netherlands) according to manufacturer’s instructions. The percentages inhibition was calculated using the values in the sera without maggot ES as 0%. The results are means and SD of four independent experiments.