Human Antibodies Can Cross Guinea Pig Placenta and Bind Its Neonatal Fc Receptor: Implications for Studying Immune Prophylaxis and Therapy during Pregnancy

Table 1

Primers used to clone single-chain, soluble guinea pig FcRn. Underlined sequences correspond to EcoRI and BglII enzymatic sites in the B2M and FCGRT primers, respectively, whereas the sequences shown in bold face correspond to overlapping sequences in the overlapping PCR reaction which allow B2M and FCGRT to join in one segment separated by three GGGS linker sequences (in italics, see text for description).

Name	Primer DNA sequence	Primer corresponding peptide sequence
B2M forward	CTGGAATTCGCCGGCTGTGGACGCCGTCCTC	W N S P A V D A V L
B2M reverse	GCCACCTCCGCCTGAACCGCCTCCACCCTTGTTTGGATCCCATTT	K W D P N K G G G G S G G G G
FCGRT forward	GGCGGAGGTGGCTCTGGCGGTGGCGGATCAGCCGAGAACCACCTGAGCCTGCTGTACC	G G G G S G G G G S A E N H L S L L Y
FCGRT reverse	CAGAGATCTGCTGCTGCCGGCGGGGCTCTCCAGCTCC	E L E S P A G S S R S L
FCGRT reverse with stop codon	CAGAGATCTCTATTAGCTGCTGCCGGCGGGGCT	S P A G S S Stop Stop R S L