Variable EBV DNA Load Distributions and Heterogeneous EBV mRNA Expression Patterns in the Circulation of Solid Organ versus Stem Cell Transplant Recipients
Figure 4
(a) Localisation of EBV DNA in PBMCs of 8 SOT patients sorted into B cells, T cells, and monocytes. As control PBMCs of a patient with NK/T lymphoma was used, indicated with an asterix. PBMCs were sorted by FACS in monocytes and B and T cells. Viral load was determined in 10e5 sorted cells. The purity of the sorted cells was higher than 85%. (b) EBV DNA load in follow-up samples of SOT patients. The arrow indicates the time point of sampling for the analysis of the viral load in the B cells, T cells, and monocytes.
