Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus
Figure 5
Comparison of plasma and intracellular GSH levels, glutathione peroxidase (GSH-Px) enzyme activity, GSH-Px isomer expression, and glutathione reductase (GSSG-R) enzyme activity in T and PMN from normal and active SLE groups. (a) Plasma GSH levels. (b) Intracellular GSH levels (μM/1 × 107 cells/mL) in T and PMN of normal and SLE patients. (c) GSH-Px (mU/1 × 107 cells/mL) enzyme activity in T and PMN of normal and active SLE group. One milliunit (mU) of GSH-Px enzyme activity is the activity that catalyzes the reduction of 1 nmol NADP+/mL/min. (d) Western blot analysis of GSH-Px isomer distribution in two cases of T and PMN from 2 normal and 2 SLE patients. Both normal PMN and SLE-PMN contain mainly monomer (25 kDa) and trimer (75 kDa) isomers rather than dimer (50 kDa) and tetramer (100 kDa) isomers. In contrast, dimer (50 kDa) and tetramer (100 kDa) isomers were the main isomers found in normal and SLE-T cells. Lanes 1 and 2 are different cases of PMN. Lanes 3 and 4 are different cases of T. (e) Comparison of GSSG-R enzyme activity in T and PMN of normal and SLE group members. (f) Intracellular GSH levels in active SLE-T before and after effective treatment. (g) Intracellular GSH levels in active SLE-PMN before and after effective treatment.
