Figure 2: Inflammatory score of IL-10−/− and IEL profile. (a) Macroscopic score of IL-10−/− mice at 6, 10, or 16 weeks of age and control age-matched wild-type mice. (b) Microscopic score of IL-10−/− mice at 6, 10, or 16 weeks of age and control age-matched wild-type mice obtained by histological analysis of the colonic tissues. (c) Villus/crypt ratio of duodenum, proximal jejunum, distal jejunum, and ileum of IL-10−/− mice at 6, or 16 weeks of age. Bars represent the mean ± SEM of 5 mice per group. Asterisks represent differences from age matched WT groups in the same intestinal segment (* or **). (d) Flow cytometry analysis with frequency of IEL isolated from 16-week-old IL-10−/− and control mice stained with fluorescent antibodies to αβTCR, γδTCR, and Thy1.2. Cells were analyzed inside the total lymphocytes gate. Bars represent the mean ± SEM of 3 mice per group. Significant difference (**) is indicated by the asterisk. n.d means non detected.