New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa

Figure 4

Production of cytokines in the intestinal mucosa during the course of intestinal inflammation. Intestines from IL-10^−/− at 6, 10 or 16 weeks of age were removed, separated into duodenum, proximal jejunum, distal jejunum, ileum and homogenized in extract buffer. Age-matched 129Sv/Ev (WT) mice were used as controls. Extract supernatant was collected for cytokine assay. IL-17A (a), IL-6 (b), TGF-β (c), and IFN-γ (d) were measured by ELISA. 𝑛=5 mice per group. Asterisks represent differences among groups in the same intestinal segment (*𝑃<0.05, **𝑃<0.01 or ***𝑃<0.001).

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