Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B
Figure 5
Interferon (IFN)-g secretion was measured in an ELISPOT assay with splenocytes isolated from C57BL/6 mice immunized with different rBCG strains after 6 and 24 weeks . Freshly isolated spleen cells were plated in duplicate at 2.5 × 105 cells per well and incubated with 2 μg/well of PPD, Ag85B, Ag85A, or culture medium control for 72 h at 37°C, 5% CO2, respectively. Results are expressed as the mean (±SD). This experiment was repeated with similar results. There was statistic difference between all groups with different stimulations except rBCG::85B versus rBCG::261 with PPD stimulation at 6 and 24 weeks.
