TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets
Figure 1
Cloning and validation of MC2/A2 and MA3/DP4 TCRαβ genes. (a) Schematic representation of how TCR DNAs have been cloned, typed for TCR-V(D)J gene usage, and tested in T cells following gene transfer. (b) TCR-V(D)J and C classification of the TCRα and β chains expressed by EB81-CTL16 and R12-C9 according to http://www.imgt.org/. The arrow before the Jα6.01 indicates a frame shift preventing surface expression of this TCR-Vα2 chain. Sequence data for human TCR-Vα2, Vα3, and Vβ28 of EB81-CTL16-derived TCR genes are available from GenBank under accession nos. EU427373, EU427374, and EU427375, respectively; and sequence data for human TCR-Vα38 and Vβ22 of R12-C9-derived TCR genes are available from GenBank under accession nos. EU427376 and EU427377, respectively.
