TCR Gene Transfer: MAGE-C2/HLA-A2 and MAGE-A3/HLA-DP4 Epitopes as Melanoma-Specific Immune Targets
Figure 3
MC2/A2 TCR is functionally expressed by primary human T cells. (a) MC2/A2 TCR T cells lyse MC2/A2 positive target cells. TCR T cells were tested in a 6 h 51Cr-release assay. The following target cells were used: MC2/A2-positive EB81-MEL-2 melanoma cells (derived from the same patient from whom the MC2 TCR was derived), pretreated or not with IFNγ, and A2-positive BSM EBV-B cells, pulsed either with gp100 or MC2 peptide (both at 10 μM final). Mock T cells did not lyse MC2/A2-positive target cells (data not shown). Effector-to-target cell ratios are indicated on the -axis and specific 51Cr-releases are indicated on the -axis. (b) MC2/A2 TCR T cells produce cytokines upon coculture with MC2/A2-positive target cells. T-cell production of IFNγ and TNFα (in pg/mL) was measured by ELISA in supernatants harvested after an 18 h coculture between T cells and the target cells described in legend to Figure (a) No cytokines were produced by T cells only or Mock T cells cocultured with MC2-positive target cells (data not shown). Measurements were performed in triplicate and expressed as mean values corrected for medium values. Data shown are from representative experiments out of 4 experiments from 2 donors with similar results.
