Host Susceptibility to Brucella abortus Infection Is More Pronounced in IFN-γ knockout than IL-12/β2-Microglobulin Double-Deficient Mice
Figure 4
IL-12 and TNF-α production induced by B. abortus in IFN-γ KO or IL-12/β2-m KO macrophages. Bone marrow from C57BL/6, IFN-γ −/−, and IL-12/β2-m−/− mouse cells were differentiated in macrophages and stimulated with B. abortus S2308 (MOI 100 : 1) or E. coli LPS (1 μg/mL). Supernatants were harvested for measuring IL-12 (a) and TNF-α (b) after 24 hrs by ELISA. Significant difference in relation to nonstimulated cells is denoted by the symbol # and an in relation to C57BL/6 mice is denoted by an asterisk (P < 0.05). Results are means ± standard deviations of experiments performed with three animals. Data shown are representative of two different experiments.
