Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
Figure 2
The apoptosis of T cells in allo-MLC stimulated with TB patient DCs was higher, and T cell proliferation was lower than that stimulated with healthy donor DCs. Date are presented in median value and whiskers (min to max). (a) After 3-day MLC stimulated with DCs of (PPD-) TB patients, the number of apoptotic CD4+ cells was significantly higher compared with MLC induced by healthy donor DCs. (b) After 3-day MLC stimulated with TB patient DCs the number of apoptotic CD8+ cells was significantly higher than that in MLC stimulated with healthy donor DCs. The number of apoptotic CD8+ cells in MLC stimulated with DCs of PPD-anergic TB patients was significantly higher than that in MLC cultures with healthy donor DCs (*) or PPD-responsive patient DCs (#). (c) The number of CD4+ cells in S and G2/M phase of cell cycle in allo-MLC stimulated with TB patient DCs was higher than that stimulated with healthy donor DCs. (d) The number of CD8+ cells in S and G2/M phase of cell cycle in allo-MLC stimulated with patient DCs and DCs of PPD-anergic patients was higher than that stimulated with healthy donor DCs.
